Conformational Changes in Hemoglobin S ( E6V) Imposed by Mutation of the Glu– Lys Salt Bridge and Detected by UV Resonance Raman Spectroscopy*
نویسندگان
چکیده
From the ‡Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, the **Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, New York 10461, the ‡‡Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, and ¶INSERM, Unité 473, 84 rue du Général Leclerc, 94276 Le Kremlin-Bicêtre, France
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Mutation of Glu-80-->Lys results in a protein C mutant that no longer requires Ca2+ for rapid activation by the thrombin-thrombomodulin complex.
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